Mouse Hippocampus Phosphoproteomic Profiles After Timed Acute Exercise

Mouse hippocampus phosphoproteomic data investigates time-of-day effects on exercise-induced signaling. The study identified 932 and 828 differentially regulated phosphosites during the rest and active phases, respectively, with only 49 overlapping sites. Data was generated by affinity enrichment and LC-MS/MS on samples from mice exercised at ZT3 or ZT15.

Use Cases

• Compare the 932 and 828 differentially regulated phosphosites between ZT3 and ZT15 exercise groups to identify time-dependent signaling.
• Analyze the 49 overlapped phosphosites on 44 proteins to find core exercise-responsive pathways independent of circadian timing.
• Predict upstream kinases using the phosphoproteomic profiles to infer exercise- and time-specific kinase activity changes.
• Investigate the 29% of differentially phosphorylated proteins associated with synapse structure or function for neuroplasticity insights.
• Examine pathways like glutamate synapse-calcium signaling-LTP modulated by timed exercise via distinct molecular nodes.

Strengths

• Quantifies 932 and 828 differentially regulated phosphosites, providing a detailed map of exercise-induced phosphorylation changes.
• Includes bioinformatic analyses such as functional enrichment, motif analysis, and kinase prediction on the phosphoproteomic data.
• Licensed under CC BY 4.0, permitting open sharing and adaptation of the 22.1 MB dataset.

Limitations

• Sample size and number of biological replicates are not specified, limiting assessment of statistical power.
• Data is restricted to a single mouse model and acute exercise protocol, reducing generalizability to other conditions or species.
• The 22.1 MB file size suggests a relatively small-scale proteomic dataset, which may limit deep learning applications.

Provenance

Source

Figshare, authored by Ping Qian.

Collection Method

Phosphoproteome conducted by affinity enrichment and liquid chromatography-tandem mass spectrometry (LC-MS/MS) on mouse hippocampus.

Time Range

Samples collected immediately after acute exercise at ZT3 (early rest phase) or ZT15 (early active phase).

Freshness

Last updated March 23, 2026.

Geography

Laboratory study, geographic location not specified.