TLN1-NGFR Interaction Data Supporting Castration-Resistant Prostate Cancer Research

A 2026 research dataset from figshare by Sixin Li, analyzing the role of Talin-1 in castration-resistant prostate cancer. The data includes results from mass spectrometry of patient serum, immunohistochemistry on 76 clinical tissue samples, and functional assays on cell lines. Findings indicate TLN1 interacts with NGFR and influences the PI3K-AKT and MAPK signaling pathways.

Use Cases

• Validate TLN1 as a biomarker for castration-resistant prostate cancer based on its upregulation in patient serum and tissues.
• Study the molecular interaction between TLN1 and NGFR using the described co-immunoprecipitation and molecular docking results.
• Investigate signaling pathway alterations (PI3K-AKT, MAPK, NF-κB) in CRPC cell lines following TLN1 knockdown.
• Analyze transcriptomic changes associated with TLN1 knockdown to identify downstream targets like NGFR.

Strengths

• Includes validation across multiple methods: mass spectrometry, immunohistochemistry on 76 tissue samples, qPCR, and Western blot.
• Functional validation performed in vitro (cell proliferation, migration, invasion assays) and in vivo (nude mouse xenograft model).
• Mechanistic exploration includes transcriptome sequencing, molecular docking, and co-immunoprecipitation experiments.

Limitations

• The primary data file is a 2.2 MB PDF, which likely contains summarized results rather than raw, machine-readable data tables.
• Row count and column-level documentation are unknown, limiting direct computational analysis.
• Data freshness should be verified as the last update date is in the future (2026-04-23).

Provenance

Source

figshare

Collection Method

Experimental data from mass spectrometry, immunohistochemistry, functional cell assays, transcriptome sequencing, and animal models.

Freshness

Last updated 2026-04-23 05:35:40