Transcriptomic Analysis of High-Altitude Cognitive Dysfunction in Mice

A 2026 study by Yu Xin investigates the molecular mechanisms of high-altitude cognitive dysfunction in mice. The dataset includes RNA-Seq results identifying 178 differentially expressed genes and validation data from qRT-PCR and Western blot experiments. It is published under a CC-BY-4.0 license on figshare.

Use Cases

• Identifying differentially expressed genes associated with hypobaric hypoxia based on RNA-Seq results.
• Validating hub gene expression changes using qRT-PCR data for genes like Vwf, Vegfa, Kdr, and Spp1.
• Analyzing pathway inhibition through Western blot protein expression data for the PI3K/AKT signaling pathway.
• Studying correlations between oxidative stress, neuroinflammation, and blood-brain barrier disruption based on described experimental assays.

Strengths

• Includes validation of RNA-Seq findings with qRT-PCR and Western blot data, suggesting multi-method verification.
• Identifies 178 differentially expressed genes (70 upregulated, 108 downregulated) and 8 hub genes, providing specific molecular targets.
• Published under a permissive CC-BY-4.0 license, allowing for reuse and redistribution.

Limitations

• The primary data file is a 2.5 MB DOCX document, which likely contains a manuscript rather than raw, structured data tables.
• Row count and column-level documentation are unknown, making it difficult to assess the dataset's structure and scale for direct analysis.
• Data is derived from a specific mouse model (male C57BL/6 J mice), which may limit generalizability to other species or conditions.

Provenance

Source

figshare, author Yu Xin.

Collection Method

Generated from a controlled laboratory study involving hypobaric hypoxia exposure, behavioral tests, histological analysis, RNA-Seq, qRT-PCR, and Western blot.

Freshness

Last updated 2026-04-23 05:36:20.