TgF344 Rats: Hippocampal Epigenetic and Microglial Changes After Treadmill Training

Aged TgF344-AD rats underwent progressive treadmill training, with hippocampal histopathology and DNA modification profiles characterized. Reduced-representation bisulfite sequencing indicated 94 genes associated with differentially modified cytosines in exercised females and 87 in exercised males. Quantitative high-throughput slide scanning fluorescence microscopy revealed female-specific reductions in neuronal and microglial density, and sex-dependent changes in plaque-associated microglial reactivity.

Use Cases

• Analyze sex-specific epigenetic changes in Alzheimer's disease models based on differentially modified cytosines and regions
• Investigate the impact of exercise on microglial morphology and reactivity state based on plaque-associated microglial data
• Study correlations between physical exertion and neuronal density based on NeuN+ cell counts
• Explore functional enrichment of pathways related to glial activation and synaptic plasticity based on protein interaction data

Strengths

• Includes sex-specific analysis, with distinct results reported for female (239 differentially modified regions) and male (216 differentially modified regions) rats
• Combines two analytical methods: reduced-representation bisulfite sequencing for epigenetic data and quantitative high-throughput slide scanning fluorescence microscopy for histopathology
• Focuses on a specific, well-defined rodent model (aged TgF344-AD rats) and intervention (progressive treadmill-training)

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• The primary data file is a DOCX document (1.2 MB), which may not be a standard format for structured data analysis

Provenance

Source

Adam J. Schuller

Collection Method

Experimental study using rodent models, likely contains data from laboratory measurements and sequencing.

Freshness

Last updated 2026-05-05 15:19:07; freshness should be verified