Data Sheet 1_Unraveling the anti-neuroinflammatory mechanisms of Cervus cucumis polypeptid

A research document details an integrated computational and experimental study on Cervus cucumis polypeptide injection (CCPI) for Alzheimer's disease. The study, authored by Xiaowei Fu and uploaded to figshare in April 2026, identifies 28 active components and 50 common targets, focusing on the IL-6/STAT3/VEGF pathway. It includes results from network pharmacology, molecular docking, molecular dynamics simulation, and in vitro validation on Aβ-induced microglial cells.

Use Cases

• Identify potential drug targets for Alzheimer's disease based on the 50 common targets identified between CCPI and AD.
• Validate molecular interactions using the described molecular docking and dynamics simulation results for IL-6.
• Study microglial polarization and neuroinflammatory pathways using the in vitro experimental results on BV2 cells.
• Explore the application of network pharmacology for traditional Chinese medicine formulations.
• Investigate the role of the HIF-1 and IL-6/STAT3/VEGF signaling pathways in neuroinflammation.

Strengths

• The study integrates four distinct methodologies: network pharmacology, molecular docking, molecular dynamics simulation, and experimental validation.
• Specific results are reported, including 28 active components, 50 common targets, and the identification of linoleic acid and IL-6 as key component-target pair.
• In vitro experiments measured concrete outcomes like cell viability (CCK-8), cytokine levels (ELISA), and protein expression (Western blot).

Limitations

• The dataset is a 2.0 MB DOCX file; the underlying raw data (e.g., docking scores, simulation trajectories, raw assay data) is not provided in a structured, machine-readable format.
• Column-level documentation is absent; field semantics must be inferred from the research narrative after download.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.

Provenance

Source

Research by Xiawei Fu, shared via figshare.

Collection Method

Data retrieved from TCMSP, Genecards, OMIM, and DrugBank databases, followed by computational analysis and lab experiments.

Freshness

Last updated 2026-04-22 05:49:22; freshness should be verified.