Sea Urchin Immune Cell Transcriptional Response to UVB-Induced DNA Damage

Purple sea urchin Strongylocentrotus purpuratus immune cells (coelomocytes) were exposed to UVB radiation to induce genotoxic stress. Transcriptional responses were measured via single-cell RNA sequencing at six hours post-exposure, identifying activation of DNA damage response, autophagy, ubiquitin signaling, and innate immune pathways. The dataset was created by Riss M. Kell and published on figshare in March 2026.

Use Cases

• Identify conserved DDR gene activation patterns based on transcriptional response data.
• Analyze cell-type-specific responses to genotoxic stress based on single-cell RNA sequencing results.
• Study the link between autophagy and DNA damage signaling based on pathway activation data.
• Compare innate immune pathway activation in invertebrates versus vertebrates based on the molecular framework.
• Investigate post-translational modification substrates in key signaling pathways based on functional assay results.

Strengths

• Includes single-cell RNA sequencing data, providing cell-type-specific resolution.
• Focuses on a long-lived, cancer-resistant model organism (Strongylocentrotus purpuratus) diverged from vertebrates ~550 million years ago.
• Data is licensed under CC-BY-4.0, allowing for open reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is 1.1 MB, suggesting a relatively small scale.

Provenance

Source

figshare

Collection Method

Single-cell RNA sequencing and functional assays (western blot) on sea urchin coelomocytes after UVB exposure.

Freshness

Last updated 2026-03-19 05:18:32; freshness should be verified.