Research Paper on IbeA-Vimentin-NF-κB Signaling in E. coli K1 Meningitis

A 441.8 KB PDF research article published by Bingliang Zhou on figshare under a CC-BY-4.0 license. The study establishes a direct mechanistic link between the IbeA virulence factor and Vimentin–NF-κB signaling in Escherichia coli K1 meningitis pathogenesis. Experimental methods include in vitro assays with human brain microvascular endothelial cells and in vivo models using neonatal rats.

Use Cases

• Analyze the role of IbeA in bacterial adhesion, invasion, and biofilm formation based on the described TEER, confocal imaging, and crystal violet assays.
• Study Vimentin dynamics and its interaction with NF-κB based on the described lipid-raft fractionation, cytoskeleton extraction, and co-immunoprecipitation methods.
• Evaluate the efficacy of glatiramer acetate as a pharmacological inhibitor based on the described in vitro and in vivo experimental results.
• Investigate bacterial dissemination and blood-brain barrier disruption based on the described neonatal rat meningitis model.

Strengths

• The dataset is a structured research article with a clear introduction, methods, results, and conclusions section.
• The study provides a novel mechanistic link between IbeA's multiple virulence functions and the VIM–NF-κB signaling pathway.
• Experimental validation includes both in vitro cell models and an in vivo neonatal rat model.

Limitations

• The dataset is a single PDF file (441.8 KB) containing a research paper, not a structured data table for direct computational analysis.
• Column-level documentation is absent; field semantics must be inferred from the text.
• Row count is unknown, which may limit suitability assessment for typical ML tasks.

Provenance

Source

figshare

Collection Method

Experimental research involving isogenic mutant generation, cell culture assays, animal models, and molecular biology techniques.

Time Range

The temporal coverage of the experimental data is not specified.

Freshness

Last updated 2026-04-16 04:19:03; freshness should be verified.

Geography

The spatial coverage of the experimental data is not specified.