Wood Smoke Particle Effects on Human Lung Epithelial Cells

A collection of experimental results on the effects of Douglas fir wood smoke particles (WSP) on a human bronchial epithelial cell line (BEAS-2B). It measures cellular events such as pro-inflammatory cytokine mRNA expression, gap junction gene expression, and polycyclic aromatic hydrocarbon (PAH) content. The data supports research into the mechanisms linking wildfire smoke exposure to adverse lung events.

Use Cases

• Analyze the relationship between WSP concentration and mRNA levels of pro-inflammatory markers like TNF, IL-6, COX-2, and IL-8.
• Investigate the correlation between WSP exposure and the expression of gap junction genes GJA1 and GJB2.
• Study the identified polycyclic aromatic hydrocarbons (PAHs) in WSP and their association with the induction of metabolizing enzymes CYP1A1 and CYP1B1.
• Examine the effect of the compound parthenolide on WSP-induced dysregulation of gap junction activity and TNF mRNA expression.
• Assess the upregulation of the growth factor epiregulin in response to WSP as a potential marker for tumor-promoting inflammation.

Strengths

• Data is derived from controlled experiments on a specific human bronchial epithelial cell line (BEAS-2B).
• Includes measurements for multiple biological endpoints, including gene expression, cytokine secretion, and chemical analysis of PAHs.
• Results are linked to a specific, noncytotoxic WSP concentration of 5 µg/mL.
• Dataset is licensed under CC BY 4.0 for open sharing and reuse.

Limitations

• The dataset is contained within a 1.7 MB DOCX file, which is a small, non-tabular format not optimized for direct computational analysis.
• Sample size and number of experimental replicates are not specified in the provided input.
• Data is limited to in vitro cell line experiments, which may not fully represent in vivo human lung responses.

Provenance

Source

figshare, authored by Deedee Romo.

Collection Method

Experimental data from laboratory studies on BEAS-2B cells exposed to Douglas fir wood smoke particles, with analysis using techniques like gas chromatography-mass spectrometry (GC/MS).

Freshness

Last updated on 2026-03-20.