Murine Sepsis Model Chromatin Remodeling in MDSCs

A high-resolution epigenetic map profiles DNA methylation and chromatin accessibility in myeloid-derived suppressor cells (MDSCs) from a murine sepsis model. The data includes young and older adult male and female mice, with unsupervised clustering identifying nine promoter classes reflecting chromatin dynamics. Transcriptomic profiling links these promoter accessibility states with gene expression.

Use Cases

• Analyze age- and sex-dependent sepsis-induced promoter opening (Classes 1-4) to identify epigenetic drivers of immune dysfunction.
• Investigate persistent promoter closure with varying DNA methylation levels (Classes 5-7) for associations with long-term myeloid suppression.
• Correlate promoter accessibility states from MAPit-FENGC data with single-cell RNA sequencing results to validate expression links.
• Examine constitutive openness post-sepsis (Classes 8, 9) to define stable epigenetic features in splenic CD11b+Gr1+ cells.

Strengths

• Data generated using MAPit-FENGC, a single-molecule assay providing simultaneous DNA methylation and chromatin accessibility profiles.
• Analysis includes both young and older adult mice of both sexes, capturing age and sex dimensions.
• Findings define nine distinct promoter classes, providing a detailed categorization of chromatin dynamics.

Limitations

• Dataset is a 2.2 MB PDF file, limiting direct computational analysis of underlying tabular or sequence data.
• Data is derived from a murine model, and direct translation to human sepsis pathophysiology may be limited.
• Sample size and specific row/column counts for underlying molecular measurements are not provided in the description.

Provenance

Source

Angel M. Charles via figshare.

Collection Method

Splenic MDSCs isolated from a murine sepsis model for MAPit-FENGC epigenetic profiling and single-cell RNA sequencing.

Freshness

Last updated March 2026.