Proof-of-Concept Immunochromatographic Strip for Staphylococci Detection in Dairy Milk

A 18.2 KB document describes the development of a colloidal gold nanoparticle-based immunochromatographic dip strip for detecting staphylococcal bacteria in dairy milk. The assay achieved a limit of detection of 10^8 CFU/mL in UHT milk, with results available within 20 minutes and strip stability demonstrated for up to 30 days. Author Chatsiri Siriwathanakul published the document on figshare in April 2026 under a CC-BY-4.0 license.

Use Cases

• Benchmarking rapid diagnostic assay performance based on the described 20-minute detection time.
• Evaluating genus-specific pathogen detection methods based on the described identification of Staphylococcus aureus and Non-Aureus Staphylococci.
• Assessing colloidal gold nanoparticle conjugation protocols based on the described use of 40-nm particles at pH 8.2.
• Studying assay stability for field deployment based on the 30-day storage stability at 25°C.

Strengths

• Document provides specific performance metrics, including a limit of detection of 10^8 CFU/mL.
• Assay validation details are included, such as testing at 10^9 CFU/mL and a 20-minute result time.
• Stability data is provided, indicating functional storage for up to 30 days at 25°C.

Limitations

• The dataset is a single 18.2 KB document; no raw experimental or validation data is included.
• Row count and column-level documentation are absent; data structure must be inferred from the text.
• The described assay has a limit of detection primarily suitable for samples with high bacterial load.

Provenance

Source

figshare

Collection Method

Research document describing assay development and validation.

Time Range

The study's temporal coverage is not specified.

Freshness

Last updated 2026-04-13 14:52:35.

Geography

The study's geographic coverage is not specified.