Modified MSRE-qPCR Method for Cervical Cancer Detection via cfDNA Methylation

Data Sheet 2 describes a modified Methylation-sensitive restriction enzyme-quantitative polymerase chain reaction (MSRE-qPCR) method for detecting circulating cell-free DNA methylation in cervical cancer. The method was applied to 24 blood samples from cervical cancer patients and 21 matched tissues, achieving 87.5% sensitivity and 90.3% specificity. The dataset, authored by Yaru Liu and last updated in May 2026, is shared under a CC-BY-4.0 license.

Use Cases

• Training a logistic regression model for cervical cancer status prediction based on a methylation panel of RXFP3-L1, RXFP3-L2, ZNF671, and SOX1.
• Benchmarking the sensitivity and specificity of a modified MSRE-qPCR method against other techniques like MethyLight.
• Developing a combinatorial methylation assay for cervical cancer evaluation using low DNA input (1.5 ng).

Strengths

• Method achieved 87.5% sensitivity and 90.3% specificity in the described study.
• The methylation panel of four genes yielded an AUC of 0.917 (95% CI: 0.830–1.000).
• Dataset is shared under a permissive CC-BY-4.0 license.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small at 146.4 KB, indicating limited scope.

Provenance

Source

figshare

Collection Method

Developed from an enhanced MSRE-qPCR method applied to patient blood and tissue samples.

Time Range

null

Freshness

Last updated 2026-05-04 04:19:05; freshness should be verified.

Geography

null