Apple Tissue-Specific Transcriptome and Alternative Splicing Atlas

Chenyang Hu published a dataset on figshare in May 2026 containing a full-length transcriptome analysis for apple (Malus domestica). The data includes 56,809 genes, 100,911 transcript isoforms, and an atlas of 29,842 alternative splicing events derived from seven apple tissues and fruit developmental stages. It was generated by integrating Oxford Nanopore Technology long-read cDNA sequencing with Illumina RNA-seq.

Use Cases

• Identify tissue-specific gene expression patterns based on data from seven apple tissues.
• Analyze alternative splicing variation using the percent spliced in (PSI) metrics mentioned in the description.
• Characterize full-length transcript isoforms discovered via long-read sequencing across fruit developmental stages.
• Study post-transcriptional regulation by examining the atlas of 29,842 alternative splicing events.

Strengths

• Integrates two sequencing technologies (ONT long-read and Illumina short-read) for improved annotation.
• Comprehensive coverage of 56,809 genes and 100,911 transcript isoforms.
• Systematic characterization of 29,842 alternative splicing events across seven AS types.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (577.5 KB), suggesting it contains processed summary results rather than raw sequencing data.

Provenance

Source

figshare

Collection Method

Integrated Oxford Nanopore Technology (ONT) long-read cDNA sequencing with Illumina RNA-seq.

Freshness

Last updated 2026-05-11 05:29:38; freshness should be verified.