Data Sheet 2: CXCL9 Expression in a Mouse Model of Neuromyelitis Optica

19.1 MB of experimental data from a study establishing murine models for neuromyelitis optica spectrum disorder (NMOSD). The dataset, authored by Liang Wang and shared under CC-BY-4.0, includes results from adoptive transfer of AQP4-specific Tfh and Th17 cells, histopathology, flow cytometry, and bulk RNA sequencing of spinal cord tissue. CXCL9 was identified as a significantly upregulated chemokine of astrocytic origin.

Use Cases

• Validate transcriptomic findings related to CXCL9 upregulation based on the bulk RNA sequencing results described.
• Correlate clinical severity scores with histopathological markers like CD3, B220, GFAP, IBA1, and MBP expression mentioned in the description.
• Analyze immune cell infiltration patterns into the central nervous system based on the flow cytometry profiling data.
• Investigate the differential pathogenic roles of AQP4-specific Tfh versus Th17 cells based on the experimental model outcomes.

Strengths

• Data originates from a controlled experimental study with defined murine models, as detailed in the methods.
• Includes multi-modal validation through histopathology, immunohistochemistry, flow cytometry, and RNA sequencing, as described.
• Shared under a permissive CC-BY-4.0 license, facilitating reuse and redistribution.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for statistical analysis.
• The dataset is 19.1 MB, which is a small scale and may contain limited sample data.

Provenance

Source

Liang Wang, via figshare.

Collection Method

Data generated from laboratory experiments involving AQP4-knockout mice, adoptive T-cell transfer, histopathology, flow cytometry, and bulk RNA sequencing.

Freshness

Last updated 2026-05-28 04:50:55; freshness should be verified.