ant(9)-Ie: Novel Spectinomycin Resistance Gene in Providencia Rettgeri P17

A 215.5 KB PDF describes the discovery and characterization of a novel resistance gene, ant(9)-Ie, in Providencia rettgeri P17. The gene, identified from wastewater on an animal farm, confers a 64-fold increase in spectinomycin MIC and has a catalytic efficiency of (8.22 ± 1.24) × 10² M⁻¹·s⁻¹. The dataset, authored by Lulu Huang and last updated in June 2026, includes results from molecular cloning, kinetic analysis, and comparative genomics.

Use Cases

• Comparative genomic analysis of aminoglycoside resistance genes based on the described conserved genetic environment.
• Kinetic modeling of enzyme activity based on the reported spectinomycin catalytic efficiency parameters.
• Phylogenetic analysis of resistance gene backgrounds based on the described sequence similarity to ANT(9)-Id.
• Studying the distribution of resistance genes in environmental versus clinical isolates based on the source comparison mentioned.

Strengths

• Includes specific kinetic parameters for the ANT(9)-Ie enzyme, such as a spectinomycin catalytic efficiency of (8.22 ± 1.24) × 10² M⁻¹·s⁻¹.
• Reports a concrete 64-fold increase in the Minimum Inhibitory Concentration (MIC) of spectinomycin for the recombinant strain.
• Provides a detailed methodological description including whole-genome sequencing, molecular cloning, and comparative genomic analysis.

Limitations

• The primary data (e.g., raw sequencing reads, full genomic assemblies) are not directly available as a structured dataset; the file is a 215.5 KB PDF.
• Row count and column-level documentation are absent, limiting suitability assessment for direct computational analysis.
• The data scope is limited to a single bacterial strain (Providencia rettgeri P17) and a specific resistance gene.

Provenance

Source

Isolated from wastewater from an animal farm.

Collection Method

Bacterial isolation, agar dilution MIC testing, molecular cloning, heterologous expression, kinetic analysis, whole-genome sequencing, and comparative genomics.

Freshness

Last updated 2026-06-02 04:28:46.