Genomic Determinants of Antifungal Activity in Streptomyces Strain STM-2

Comprising genomic analysis results for Streptomyces melanosporofaciens STM-2, a strain isolated from Chiayi County, Taiwan. The study identified 52 biosynthetic gene clusters (BGCs) and specific genes for antifungal enzymes like chitinases and proteases. It includes results from comparative genomics and AlphaFold2 protein modeling.

Use Cases

• Analyze the distribution of 52 predicted biosynthetic gene clusters (BGCs) across types like PKS, NRPS, and terpenes for natural product discovery.
• Investigate the correlation between identified antifungal enzyme genes (e.g., chitinases, β-1,3-glucanases) and the strain's biocontrol potential.
• Use the high-GC (71%) chromosome characteristic and ANI/dDDH scores for comparative pangenomic analysis with other Streptomyces strains.
• Validate in silico structural models of unique gene products generated by AlphaFold2 with high pLDDT scores for functional annotation.

Strengths

• Identifies 52 biosynthetic gene clusters (BGCs) predicted by antiSMASH, including diverse types like PKS, NRPS, RiPPs, and terpenes.
• Includes high-confidence structural evidence from AlphaFold2 protein models with very high pLDDT scores.
• Provides evolutionary context through comparative pangenomic analysis supported by ANI and dDDH scores.
• Focuses on a specific, novel Streptomyces melanosporofaciens strain (STM-2) with a characterized high-GC (71%) linear chromosome.

Limitations

• The dataset is very small at 36.6 KB, indicating limited scope, likely containing summary results rather than raw genomic sequences.
• Rows and columns are unknown, preventing assessment of data volume and feature granularity.
• Findings are specific to a single bacterial strain (STM-2), limiting generalizability without comparative data from other strains.

Provenance

Source

figshare, authored by Muhammed Opoku Gyamfi.

Collection Method

Integrated approach involving genome sequencing, annotation, antiSMASH mining, comparative genomics, and AlphaFold2 modeling.

Freshness

Last updated March 20, 2026.

Geography

Chiayi County, Taiwan (source of the STM-2 strain isolation).