Data Sheet 2: GPR68 Inhibition and Ferroptosis in DIPG Cell Lines

A research data sheet details experiments on GPR68 inhibition in Diffuse Intrinsic Pontine Glioma (DIPG) cell lines. The document, authored by Leif R. Neitzel and shared under a CC-BY-4.0 license, reports results from viability assays, ferroptosis marker measurements, and genetic validation experiments. It was last updated on May 13, 2026, and is available as a 93.4 KB PDF file.

Use Cases

• Validate GPR68 as a therapeutic target for DIPG based on cell viability assay results.
• Study ferroptosis induction in cancer cells based on glutathione levels and lipid peroxidation markers.
• Compare drug sensitivity between DIPG cell lines and normal human astrocytes based on LD50 values.
• Investigate the role of ATF4 and SLC7A11 in ferroptotic cell death based on qRT-PCR and overexpression experiments.

Strengths

• Includes specific LD50 values for drug sensitivity (e.g., NHAs: 3.84 µM, DIPG cells: ≤ 0.79 µM).
• Describes validation using multiple DIPG cell lines (SF7761, DIPG36, DIPG007) and rescue experiments.
• Provides mechanistic insights through genetic knockdown and overexpression of GPR68 and ATF4.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is a single 93.4 KB PDF file, which is a very limited data scope.

Provenance

Source

figshare

Collection Method

Experimental research involving cell culture assays, qRT-PCR, and genetic manipulation.

Freshness

Last updated 2026-05-13 04:48:20; freshness should be verified.