Ocimum Sanctum Nanoemulsion Metabolomic and Bioactivity Data

Laying out results from a study characterizing an Ocimum sanctum nanoemulsion, including physicochemical properties and multi-bioactivity assays. It reports droplet size (51–73 nm), cytotoxicity IC50 values (13–25 μg/mL against four cancer cell lines), and antioxidant SC50 values (16.4 μg/mL). The data was generated by Mervat S. Mohamed and published in 2026.

Use Cases

• Analyze the relationship between nanoemulsion droplet size, polydispersity index (PDI), and cytotoxicity IC50 values across Caco-2, HepG2, MDA-MB-231, and A549 cell lines.
• Model the biphasic release profile data to predict compound delivery kinetics over an eight-day period.
• Validate network pharmacology predictions by correlating identified metabolite profiles with experimentally measured reductions in TNF-α and IL-6 secretion in LPS-stimulated RAW 264.7 macrophages.
• Compare the antibacterial inhibition zones (up to 14.8 mm) and antioxidant SC50 values between the nanoemulsion and the crude extract.

Strengths

• Reports specific, quantified bioactivity results, including IC50 values for four cancer cell lines and SC50 for antioxidant activity.
• Includes detailed physicochemical characterization metrics such as droplet size (51–73 nm), PDI (0.264), and zeta potential (-42.1 mV).
• Integrates multiple analytical methods: metabolomic profiling (GC–MS, LC–QTOF–MS/MS), biological assays, and network pharmacology.

Limitations

• Data is presented in a static PDF (15.9 MB), limiting direct computational analysis and requiring extraction for tabular use.
• The study is described as preliminary, with findings based on in vitro models requiring further in vivo validation.
• Sample size or replicate counts for experimental measurements are not specified in the provided description.

Provenance

Source

figshare, authored by Mervat S. Mohamed.

Collection Method

Experimental study involving nanoemulsion preparation, metabolomic profiling, and in vitro bioactivity assays.

Freshness

Last updated in March 2026.