Data Sheet 2_Laboratory tests for bovine respiratory bacteria and antimicrobial resistance

Western Canadian beef feedlots provided 760 nasopharyngeal swabs for a field evaluation of diagnostic tests. The study compares traditional culture, long-read metagenomic sequencing, and recombinase polymerase amplification for detecting key bacterial pathogens and antimicrobial resistance genes. Simon J. G. Otto authored the work, which was last updated in May 2026.

Use Cases

• Compare diagnostic test performance (sensitivity, specificity) for bovine respiratory disease pathogens based on the described Bayesian latent class model analysis.
• Evaluate the potential of long-read metagenomic sequencing for antimicrobial stewardship based on its field implementation described in the results.
• Analyze detection patterns of antimicrobial resistance genes across different age cohorts of feedlot cattle as mentioned in the methods and results.

Strengths

• Contains results from 760 respiratory samples collected from commercial feedlots.
• Provides estimates of clinical diagnostic performance (sensitivity, specificity) for three different laboratory tests.
• Represents a large-scale field evaluation of a long-read metagenomic sequencing protocol for this application.

Limitations

• Row count and column-level documentation are unknown, which limits suitability assessment.
• The primary data file is a 1.5 MB PDF, which may require extraction of underlying tabular data for analysis.

Provenance

Source

Simon J. G. Otto via figshare.

Collection Method

Nasopharyngeal swabs collected by private veterinarians as part of Canadian surveillance, with laboratory testing including culture, sequencing, and molecular assays.

Freshness

Last updated 2026-05-20 05:23:52.

Geography

Western Canadian beef feedlots.