Metabolomic Profiling of Lipid Reprogramming During 3T3-L1 Adipogenesis

170 differential metabolites were identified between pre-differentiation and 4-day differentiation stages in murine 3T3-L1 cells. The data, published by Nanping Shi under a CC-BY-4.0 license, profiles lipid changes like triglycerides and phosphatidylcholines across an 8-day differentiation period. It was last updated on 2026-05-26.

Use Cases

• Modeling lipid metabolic pathway dynamics based on the 170 identified differential metabolites.
• Investigating the role of glycerolipid and glycerophospholipid metabolism in adipogenesis as highlighted by KEGG enrichment.
• Correlating metabolite changes with gene expression using the correlation network analysis mentioned in the results.
• Studying insulin resistance pathways during cell differentiation based on the pathway analysis results.

Strengths

• Quantifies 170, 246, and 124 differential metabolites between three distinct differentiation time points.
• Focuses on specific lipid classes like triglycerides and phosphatidylcholines, providing detailed metabolic insights.
• Data is associated with a published research methodology involving UPLC-MS analysis and KEGG pathway enrichment.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 55.5 KB, indicating limited scope.

Provenance

Source

Nanping Shi via figshare

Collection Method

Metabolites were extracted from cultured murine 3T3-L1 cells at three differentiation stages and analyzed by ultra-performance liquid chromatography coupled with mass spectrometry.

Time Range

Covers an 8-day cell differentiation period.

Freshness

Last updated 2026-05-26 05:52:38; freshness should be verified.