QobuR Model: Microbial Effects on Corneal Epithelium Gene Expression

Noelia Blanco-Agudín published a dataset on figshare in 2026. It contains gene expression data from a three-dimensional in vitro corneal epithelium model, QobuR, following co-culture with specific bacteria and a microbial consortium. The data includes qRT-PCR results and immunohistochemical analyses measuring changes in proteoglycan and glycosaminoglycan biosynthesis pathways.

Use Cases

• Analyze transcriptional responses of corneal epithelial cells to specific bacterial species based on qRT-PCR data mentioned in the description
• Compare gene expression patterns induced by individual microbes versus a mixed consortium based on the described experimental conditions
• Investigate changes in heparan sulfate and chondroitin sulfate sulfation enzymes based on the specific gene expression patterns identified
• Validate three-dimensional epithelial models for incorporating host-microbe interaction components based on the study's conclusions

Strengths

• Data is derived from a controlled in vitro model designed to reproduce complex cellular interactions.
• Analysis includes both qRT-PCR and immunohistochemical validation as stated in the methods.
• Dataset is licensed under CC-BY-4.0, allowing for reuse and modification.

Limitations

• Dataset size is 231.2 KB, indicating a limited scope of data.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

Noelia Blanco-Agudín via figshare

Collection Method

Gene expression analysis via qRT-PCR and immunohistochemistry on a three-dimensional corneal epithelium model following microbial co-culture.

Freshness

Last updated 2026-05-28 04:50:33; freshness should be verified.