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Phylogenetic characterization based on internal transcribed spacer (ITS), elongation factor (TEF), and β-tubulin (TUB) regions identified three Neopestalotiopsis species causing disease outbreaks in Spain. Pathogenicity was confirmed by inoculating two representative isolates of each species onto three strawberry cultivars: ‘Rociera FNM’, ‘Palmeritas’, and ‘Plared 15105’. The study, authored by Patricia López and last updated in May 2026, corrects previous misidentifications and provides molecular tools for pathogen diagnosis.
License is CC-BY-4.0. The primary data is contained within a PDF document, which may require extraction for computational analysis.