Pharmacological and Genetic Modulation of IL-32 Expression in Intestinal Epithelial Cells

A 318.8 KB PDF research article by Etiene Moreira Gabriel, last updated in May 2026, describes experimental results on cytokine regulation and HIV-1 latency. The study investigates the impact of IL-22, ATRA, and RGZ on IL-32 expression in HT-29 intestinal epithelial cells and uses CRISPR/Cas9 knockout models to assess effects on HIV-1 outgrowth from co-cultured CD4+ T-cells of ART-treated individuals.

Use Cases

• Analyzing the effects of pharmacological agents (IL-22, ATRA, RGZ) on IL-32 isoform expression based on the described experimental results.
• Investigating the relationship between intestinal epithelial cell signaling and HIV-1 reservoir reactivation based on the co-culture viral outgrowth assay methodology.
• Studying transcriptional profiles of IL-32 knockout cell lines under TNF exposure based on the whole-genome RNA-Sequencing analysis mentioned.

Strengths

• Data is associated with a detailed, peer-reviewed-style methodology including CRISPR/Cas9 editing and RNA-Seq.
• The dataset is openly licensed under CC-BY-4.0 for reuse.

Limitations

• The primary data file is a 318.8 KB PDF, which likely contains summarized results rather than the underlying raw experimental data.
• Row count and column-level documentation are unknown, limiting suitability assessment for direct computational analysis.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare, author Etiene Moreira Gabriel.

Collection Method

Experimental research involving cell culture, cytokine stimulation, CRISPR/Cas9 gene editing, and viral outgrowth assays.

Freshness

Last updated 2026-05-26 07:48:55; freshness should be verified.