Data from a study identifying phosphatase PTP-4 as a negative regulator of innate immunity in Caenorhabditis elegans. The research involved a genome-wide RNAi screen of 166 phosphatase genes, yeast two-hybrid screening, and small-molecule compound screening. The dataset was authored by Jing Xie and last updated on 2026-06-01.
Use Cases
- Validate phosphatase regulators of the p38 MAPK pathway based on the genome-wide RNAi screen results.
- Model protein-protein interactions between PTP-4 and ATLN-1 based on yeast two-hybrid and Co-IP data.
- Screen for small-molecule inhibitors of host defense checkpoints based on the compound screening methodology.
- Analyze the structural basis of compound-target interaction based on Surface Plasmon Resonance and molecular docking data.
Strengths
- Data is derived from a genome-wide RNAi screen covering 166 phosphatase genes.
- Includes biochemical validation with a reported binding affinity (KD = 28.69 μM) for a key interaction.
- Released under a permissive CC-BY-4.0 license.
Limitations
- Row count is unknown, which may limit suitability assessment.
- Column-level documentation is absent; field semantics must be inferred after download.
- The dataset is small (20.5 KB), indicating limited scope or summary-level data.
Provenance
- Source
- figshare
- Collection Method
- Genome-wide RNAi screen, yeast two-hybrid screening, Co-IP, mutagenesis, and small-molecule screening.
- Freshness
- Last updated 2026-06-01 04:21:46; freshness should be verified.