Proteomic Analysis of Dimorphic Sperm in Pieris Rapae

Figshare hosts a proteomic dataset from Melissa Plakke, characterizing dimorphic sperm in the cabbage white butterfly, Pieris rapae. The data includes over 1,600 identified proteins, with differential abundance analyses revealing functional enrichments for eupyrene and apyrene sperm morphs.

Use Cases

• Perform differential abundance analysis on the over 1,600 identified proteins to compare eupyrene and apyrene sperm proteomes.
• Investigate evolutionary dynamics by comparing homologous sperm protein sets between Pieris rapae, Danaus plexippus, and Manduca sexta.
• Analyze protein functional enrichments, such as those linked to ion transport in eupyrene sperm or mitochondrial functions in apyrene sperm.

Strengths

• Identifies over 1,600 proteins, nearly doubling previous reports for other lepidopteran species.
• Provides differential abundance analyses distinguishing protein functions between the two sperm morphs.
• Includes comparative homology analyses with two other Lepidoptera species.

Limitations

• Dataset is very small at 26.3 KB, indicating limited scope and likely a summary or processed results table rather than raw spectral data.
• Unknown row and column counts prevent assessment of data granularity and feature completeness.

Provenance

Source

Figshare, author Melissa Plakke.

Collection Method

Proteomes characterized using high-resolution, label-free mass spectrometry.

Time Range

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Freshness

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Geography

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