Bovine Dendritic Cell Transcriptomes Before and After BCG Vaccination

Rachrapee Sukmak's 2026 dataset contains single-cell RNA sequencing data from 20,761 individual immune cells from three Bos taurus calves. The data captures transcriptomic profiles before and after BCG vaccination, with cells clustered into dendritic cell subsets, monocytes, T-cells, B-cells, and NK cells. Each cell expresses an average of 3,036 genes.

Use Cases

• Identify cDC1 and cDC2 dendritic cell subsets using expression levels of marker genes like ENSBTAG00000056208, STX4, NEBL for cDC1 and FN1, PSPH, FGL2 for cDC2.
• Analyze gene expression signatures for antigen presentation, migration, and maturation in dendritic cell subsets post-BCG vaccination.
• Compare transcriptomic shifts between pre- and post-vaccination states across 20,761 cells to study metabolic alterations in cDC1 versus inflammatory responses in cDC2.
• Cluster single-cell transcriptomes to validate immune cell populations (ALDCs, monocytes, T-cells, B-cells, NK cells) in bovine afferent lymph.

Strengths

• High-resolution single-cell data from 20,761 individual cells.
• Each cell profile includes an average of 3,036 expressed genes for detailed transcriptomic analysis.
• Paired pre- and post-vaccination samples from 3 animals enable within-subject comparisons.

Limitations

• Small sample size of only 3 calves (n=3), limiting statistical power and generalizability.
• The 2.6 MB file size suggests aggregated or processed results, not raw sequencing reads, which constrains re-analysis depth.
• Data is specific to Bos taurus calves, with uncertain applicability to other species or age groups.

Provenance

Source

Rachrapee Sukmak via figshare.

Collection Method

Single-cell RNA sequencing (scRNA-seq) of afferent lymph dendritic cells captured from skin.

Time Range

Samples collected before and after BCG vaccination (specific dates not provided).

Freshness

Last updated April 7, 2026.

Geography

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