Human Carotid Atherosclerotic Plaque Spatial Metabolomics and Single-Cell Data

Human carotid atherosclerotic plaque tissues were collected via carotid endarterectomy for metabolomic and spatial analysis. The study identified 74 differential metabolites, with arachidonic acid upregulated in unstable plaques and involved in 22 metabolic pathways. It provides spatial evidence for the linoleic acid–arachidonic acid–leukotriene D4 metabolic axis and its association with macrophage phenotypes.

Use Cases

• Analyze the spatial distribution of the 74 differential metabolites, particularly arachidonic acid, within plaque tissues using mass spectrometry imaging data.
• Investigate the association between upregulated arachidonic acid levels and macrophage phenotypes identified through single-cell sequencing of ELOVL5 and ALOX5 expression.
• Validate the predictive capability of arachidonic acid for plaque instability using the provided ROC curve analysis from the metabolomic study.

Strengths

• Identifies 74 differential metabolites from human carotid atherosclerotic plaque tissues.
• Provides direct spatial evidence for the linoleic acid–arachidonic acid–leukotriene D4 metabolic axis within plaques.
• Integrates multiple analytical techniques: metabolomics, spatial metabolomics, and single-cell transcriptomics.

Limitations

• Primary data (e.g., raw spectra, single-cell matrices) may not be included, as the main file is a 2.0 MB PDF research article.
• Sample size and specific row/column counts for underlying datasets are not provided in the description.
• Geographic or demographic details of the human tissue donors are not specified, limiting generalizability.

Provenance

Source

Jiaxin Wan via figshare.

Collection Method

Human carotid artery atherosclerotic plaque tissues collected via carotid endarterectomy (CEA), analyzed with metabolomics, spatial metabolomics (MALDI-MSI), and single-cell transcriptomics.

Freshness

Last updated 2026-03-25.

Geography

Human carotid atherosclerotic plaques; specific geographic region not stated.