Rice GA2ox Gene Family: CRISPR Mutant Phenotypes for Nine Paralogs

A 2026 study by Kun-Ting Hsieh presents systematic CRISPR/Cas9 mutagenesis of nine gibberellin 2-oxidase (GA2ox) genes in rice. The research includes 36 edited rice lines, from single- to quadruple-gene knockouts, and analyzes their effects on agronomic traits like plant height, grain number, and fertility. The findings reveal hierarchical and partially redundant regulatory relationships among dominant and minor gene paralogs.

Use Cases

• Study functional redundancy and dominance hierarchies within plant multigene families based on systematic knockout analysis.
• Model the genetic regulation of gibberellin pathways and their impact on agronomic traits like plant height and grain yield.
• Identify candidate genes for crop improvement by analyzing the phenotypic effects of specific OsGA2ox paralog knockouts.

Strengths

• Includes data from 36 systematically generated CRISPR/Cas9-edited rice lines.
• Explicitly compares single-, double-, triple-, and quadruple-gene knockout mutants for functional analysis.
• Published under a permissive CC-BY-4.0 license for reuse.

Limitations

• The primary data format is a 161.7 KB PDF, which likely contains summarized results rather than raw, machine-readable data tables.
• Row and column counts are unknown, limiting assessment of data scale and structure.
• The description lacks specific column-level documentation for any underlying tabular data.

Provenance

Source

figshare

Collection Method

Data generated via CRISPR/Cas9 gene editing of rice lines and subsequent phenotypic analysis.

Freshness

Last updated 2026-05-07 05:42:21.