Single-Cell Multi-Omics Analysis of Hepatocellular Carcinoma and Immune Checkpoint Therapy

Integrated single-cell RNA-seq data from GEO and Mendeley, analyzed to investigate metabolic reprogramming in hepatocellular carcinoma before and after immune checkpoint inhibitor therapy. The dataset, created by Xin Li and last updated in April 2026, includes results from computational analyses like Seurat, AUCell, and pySCENIC, and a prognostic model developed from TCGA data. It supports findings on pyruvate metabolism dysregulation and the therapeutic potential of LDHA inhibition combined with PD-1 blockade.

Use Cases

• Developing prognostic models for hepatocellular carcinoma based on pyruvate metabolism gene signatures.
• Analyzing cell-cell communication signaling pathways like SPP1–CD44 and APOA1–ABCA1 in the tumor microenvironment.
• Investigating transcription factor activity (e.g., MYC, SP5) in cancer cells post-immune checkpoint therapy.
• Studying pseudotime trajectories for hepatocyte differentiation states from single-cell data.
• Validating in vitro and in vivo therapeutic effects of LDHA inhibitors combined with PD-1 antibodies.

Strengths

• Data integrates multiple analysis outputs (Seurat, AUCell, pySCENIC, CellChat, Monocle) into a single 3.2 MB CSV file.
• Includes results from functional validation assays (CCK8, Transwell) and in vivo xenograft experiments.
• Prognostic model is derived from TCGA data using Cox and Lasso regression.
• Released under a permissive CC-BY-4.0 license.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Integrated analysis of public single-cell RNA-seq data from GEO and Mendeley, combined with TCGA data analysis and experimental validation.

Time Range

Temporal coverage of the underlying source data is not specified.

Freshness

Last updated 2026-04-13 05:21:19; freshness should be verified.

Geography

Spatial coverage is not specified.