Time-Resolved Haemocyte Transcriptomics in Mythimna separata Challenged by Nematodes

Time-course RNA sequencing data examines the cellular and transcriptional responses of haemocytes in the lepidopteran insect Mythimna separata to living nematodes versus inert particles. The dataset, authored by Masaya Ono and shared under CC-BY-4.0, compares responses to polystyrene beads and the free-living nematode Caenorhabditis elegans. It was last updated on May 12, 2026.

Use Cases

• Compare temporal gene expression patterns based on the time-resolved RNA sequencing described.
• Analyze differential activation of immune pathways based on the mention of Toll signaling pathway engagement.
• Study haemocyte encapsulation responses based on the described assays comparing living nematodes to inert beads.
• Identify regulatory and membrane-related processes based on the enrichment terms noted in the results.

Strengths

• Dataset provides a direct comparison of haemocyte responses to two distinct challenge types: living nematodes and inert beads.
• Analysis includes a time-resolved component, tracking transcriptional changes over a course of events.
• The dataset is shared under a permissive CC-BY-4.0 license for reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small in scale, at 1017.0 KB, indicating limited data volume.

Provenance

Source

figshare

Collection Method

Haemocytes were collected following injection treatments, and RNA sequencing was used.

Freshness

Last updated 2026-05-12 16:40:11; freshness should be verified.