Zebrafish Larvae Transcriptomic Profiling Under Simulated Microgravity

Data Sheet 2 contains transcriptomic data from a study by Juan D. Carvajal-Agudelo, uploaded to figshare on 2026-05-04. The dataset profiles gene expression in zebrafish larvae exposed to simulated microgravity at 6, 12, 18, and 24-hour intervals, focusing on the RANKL/RANK/OPG pathway and bone-related genes. The 39.5 KB XLSX file includes results from RT-qPCR and transcriptomic analyses revealing changes in osteoblast and osteoclast activity.

Use Cases

• Analyzing time-series gene expression changes in bone-related pathways based on the described 6-24 hour exposure intervals.
• Investigating the RANKL/RANK/OPG signaling pathway's role in bone resorption under simulated microgravity conditions.
• Studying coordinated modulation of bone formation and resorption genes based on the described regulatory clusters.
• Exploring the involvement of WNT, BMP, HIPPO, and MAPK signaling pathways in skeletal regulation as mentioned in the functional enrichment results.

Strengths

• Data is focused on a specific biological model (zebrafish larvae) and perturbation (simulated microgravity).
• Includes a clear temporal dimension with measurements at 6, 12, 18, and 24 hours post-exposure.
• Released under a permissive CC-BY-4.0 license for reuse.

Limitations

• Row count and column-level documentation are unknown, which may limit suitability assessment.
• The dataset is very small at 39.5 KB, indicating limited scope or a summary dataset rather than raw sequencing data.
• Description metadata is limited; actual data structure and quality require manual inspection after download.

Provenance

Source

Juan D. Carvajal-Agudelo via figshare

Collection Method

Transcriptional analysis via RT-qPCR and transcriptomic profiling of zebrafish larvae exposed to simulated microgravity using a Random Positioning Machine.

Time Range

Experimental time points at 6, 12, 18, and 24 hours post-exposure.

Freshness

Last updated 2026-05-04 09:59:35; freshness should be verified.