Data Sheet 3_16S rRNA gene sequencing-based preliminary study on the differences in the mi

A preliminary study of 72 saliva and dental plaque samples from 36 children aged 3–5 years, grouped by arrested caries, rampant caries, or caries-free status. Microbial DNA was sequenced for the V3-V4 region of the 16S rRNA gene using the Illumina MiSeq platform. The dataset, created by Meimei Li and last updated in April 2026, compares microbial community structures and identifies potential biomarkers associated with different caries states.

Use Cases

• Compare alpha and beta diversity metrics between saliva and plaque microbiomes based on the described analysis.
• Identify differentially abundant bacterial taxa associated with arrested versus rampant caries based on the Kruskal–Wallis test results.
• Validate putative species-level biomarkers for specific clinical caries states mentioned in the results.
• Investigate the link between plaque dysbiosis and caries activity suggested by the study's conclusion.

Strengths

• Includes 72 sequenced samples from a controlled cohort of 36 children.
• Analysis performed with established bioinformatics tools (QIIME2) and reference database (SILVA).
• Statistical analysis includes Benjamini-Hochberg correction for multiple testing.

Limitations

• Row count and column-level documentation are unknown, limiting suitability assessment.
• The dataset is small (2.2 KB), indicating a limited scope or summary-level data.
• Geographic origin of the 36-child cohort is unspecified, which may limit generalizability.

Provenance

Source

figshare, author Meimei Li.

Collection Method

16S rRNA gene sequencing (V3-V4 region) on Illumina MiSeq platform of non-stimulated saliva and dental plaque samples.

Freshness

Last updated 2026-04-17 05:21:40; freshness should be verified.