Murine Sepsis Model Chromatin Accessibility Data

A high-resolution epigenetic map profiles DNA methylation and chromatin accessibility in myeloid-derived suppressor cells from young and older adult male and female mice following sepsis. The dataset includes unsupervised clustering results identifying nine promoter classes reflecting chromatin dynamics. Data was generated using the MAPit-FENGC single-molecule assay and single-cell RNA sequencing.

Use Cases

• Analyze age- and sex-dependent sepsis-induced promoter opening (Classes 1-4) to identify epigenetic drivers of immune dysfunction.
• Investigate persistent promoter closure with varying DNA methylation levels (Classes 5-7) in splenic CD11b+Gr1+ cells.
• Correlate promoter accessibility states (Classes 8, 9) from MAPit-FENGC data with gene expression from single-cell RNA sequencing.
• Examine chromatin remodeling in myeloid-derived suppressor cells to generate hypotheses on age- and sex-associated epigenetic states post-sepsis.

Strengths

• Data generated using MAPit-FENGC, a single-molecule assay providing simultaneous DNA methylation and chromatin accessibility profiles.
• Includes analysis across four biological variables: young/older adult and male/female mice.
• Unsupervised clustering defined nine distinct promoter classes, providing a structured view of chromatin dynamics.
• Transcriptomic data from single-cell RNA sequencing corroborates the epigenetic promoter states.

Limitations

• Data is derived from a murine model; direct translation to human sepsis pathophysiology may be limited.
• The 15.1 MB file size suggests the dataset is a processed summary or publication, not the underlying raw sequencing data.
• Sample size and specific row/column counts for the underlying molecular measurements are not provided.

Provenance

Source

Angel M. Charles via figshare.

Collection Method

MAPit-FENGC assay and single-cell RNA sequencing on splenic MDSCs isolated from a murine sepsis model.

Time Range

null

Freshness

Last updated March 2026.

Geography

Laboratory study (murine model).