Data Sheet 3_Discovery and characterization of a novel chromosomally encoded aminoglycosid

A 116.2 KB PDF describes the discovery and characterization of a novel chromosomally encoded aminoglycoside resistance gene, ant(9)-Ie, in Providencia rettgeri P17. The gene was identified from wastewater bacteria isolated from an animal farm and confers a 64-fold increase in spectinomycin resistance. The research, authored by Lulu Huang and last updated in June 2026, includes functional validation, kinetic analysis, and comparative genomic studies of the gene and its conserved genetic environment.

Use Cases

• Comparative genomic analysis of antimicrobial resistance genes based on the described conserved genetic environment of ant(9)-Ie.
• Kinetic modeling of enzyme efficiency based on the reported catalytic efficiency (kcat/Km) of the ANT(9)-Ie protein.
• Phylogenetic analysis of resistance gene evolution based on the amino acid sequence similarity data to ANT(9)-Id.
• Studying the distribution and host range of resistance genes based on the finding that ant(9)-Ie homologs were found only in Providencia strains.

Strengths

• Includes specific kinetic parameter for the novel enzyme: spectinomycin catalytic efficiency of (8.22 ± 1.24) × 10^2 M^−1·s^−1.
• Provides a concrete measure of phenotypic resistance: a 64-fold increase in the minimum inhibitory concentration (MIC) of spectinomycin.
• Offers comparative genomic context, noting the gene's presence only in Providencia strains with a conserved genetic environment.

Limitations

• The dataset is a 116.2 KB PDF, which is a small document; the underlying genomic sequence data is not directly provided.
• Row count and column-level documentation are absent; data must be extracted from the publication text.
• The primary data source is a single bacterial strain (P. rettgeri P17) from a specific wastewater source, limiting generalizability.

Provenance

Source

Bacteria isolated from wastewater from an animal farm.

Collection Method

Isolation by plate streaking, MIC determination by agar dilution, gene function elucidated via molecular cloning and heterologous expression, genomic analysis via whole-genome sequencing.

Freshness

Last updated 2026-06-02 04:28:45.