Gut Bacteria Diversity and Insecticide Resistance in Stegomyia aegypti from Puducherry

35 culturable gut bacterial isolates were characterized from α-cypermethrin-resistant and susceptible Stegomyia aegypti mosquitoes collected in Puducherry, India. The study by Mahima K Mani, published on figshare in April 2026, found resistant populations had greater bacterial diversity and load, dominated by Bacillota and Pseudomonadota. Antibiotic suppression of gut microbiota increased mortality upon insecticide exposure, suggesting a symbiont-mediated resistance mechanism.

Use Cases

• Analyzing the association between gut microbial diversity and insecticide resistance based on bacterial isolate counts from resistant and susceptible populations.
• Modeling the impact of antibiotic treatment on mortality rates using data from vancomycin, gentamicin, and streptomycin experiments.
• Investigating bacterial taxa contributing to resistance based on the reported dominance of Bacillaceae and Enterobacteriaceae in resistant mosquitoes.
• Studying in vitro bacterial degradation of alpha-cypermethrin using growth data from isolates like Enterobacter hormaechei and Bacillus spp.

Strengths

• Includes 35 identified bacterial isolates with clear categorization into 26 from resistant and 9 from susceptible populations.
• Provides quantitative results for antibiotic treatment efficacy, reporting mortality increases of 97%, 95%, and 92% for specific antibiotics.
• Links microbial metrics (diversity, load, taxonomic composition) directly to a confirmed phenotypic resistance status in field populations.

Limitations

• Row count is unknown, which may limit suitability assessment for large-scale statistical modeling.
• Column-level documentation is absent; field semantics must be inferred after download from the PDF.
• Data may reflect geographic bias inherent to the collection from three locations in Puducherry, India.

Provenance

Source

Mahima K Mani via figshare.

Collection Method

Mosquito larvae were collected from field sites, reared, and tested for insecticide susceptibility following WHO guidelines. Gut bacteria were isolated, identified via MALDI-TOF MS and 16S rRNA sequencing, and tested in vitro.

Time Range

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Freshness

Last updated 2026-04-14 05:28:59; freshness should be verified.

Geography

Puducherry, India.