SA-22: SIRT3 Interaction and Cardiac Mitochondrial Response Data

Experimental data from 2026 investigates the cardioprotective synthetic analog SA-22 as a positive allosteric modulator of SIRT3. The dataset likely contains results from thermal shift assays, fluorogenic activity assays, and measurements of mitophagy and mitochondrial respiration in H9c2 cells subjected to hypoxia-reoxygenation injury. It supports research into mitochondrial homeostasis and the therapeutic potential of epoxylipids in cardiovascular disease.

Use Cases

• Validating SIRT3 ligand binding based on SYPRO Orange thermal shift assay results.
• Analyzing SIRT3 catalytic activity modulation using fluorogenic substrate assay data.
• Modeling hypoxia-reoxygenation injury effects on mitophagy via mito-Keima reporter measurements.
• Assessing mitochondrial respiratory function using high-resolution respirometry (Oroboros-O2K) data.
• Investigating structure-activity relationships of epoxylipid analogs through point mutagenesis experiments on residue SER149.

Strengths

• Includes data from multiple experimental techniques: thermal shift assays, fluorogenic activity assays, cell-based hypoxia models, and respirometry.
• Released under a permissive CC-BY-4.0 license, facilitating reuse and sharing.
• Cross-platform presence on figshare indicates established curation and importance.

Limitations

• Column names and row counts are not provided, limiting understanding of the data structure.
• File sizes differ between entries (15,256,045 and 10,943,536 bytes), suggesting potential versioning or content differences not explained.
• Metadata is sparse; organization and specific data formats within the ZIP archive are unknown.

Provenance

Source

Joshua W. Kranrod

Collection Method

Experimental data from in vitro assays and H9c2 cell line models of hypoxia/reoxygenation injury.

Freshness

Last updated on 2026-06-02.