Data Sheet 3: SIRT3 Ligand Binding and Cardiac Cell Assay Results

Joshua W. Kranrod published this dataset on figshare in 2026. It contains experimental results from a study investigating the interaction between a synthetic fatty acid analog SA-22 and the mitochondrial protein SIRT3. The data likely includes measurements from thermal shift assays, catalytic activity tests, and cell-based assays of mitophagy and respiration under hypoxic conditions.

Use Cases

• Validate SIRT3 ligand binding based on SYPRO Orange thermal shift assay results.
• Analyze SIRT3 catalytic activity modulation based on fluorogenic substrate assay data.
• Model the impact of hypoxia/reoxygenation injury on cardiac cell mitophagy based on mito-Keima reporter measurements.
• Assess changes in mitochondrial respiration based on high-resolution respirometry (Oroboros-O2K) data.

Strengths

• Data is associated with a detailed methodological description including specific inhibitor concentrations (e.g., 30 µM NAM, 50 µM 3-TYP).
• Experimental design includes point mutagenesis to confirm the involvement of a specific residue (SER149).
• Released under a permissive CC-BY-4.0 license for reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 10.4 MB file size suggests a relatively small dataset.

Provenance

Source

figshare

Collection Method

Experimental data from biochemical assays (thermal shift, fluorogenic substrate) and cell culture models (H9c2 cells under hypoxia/reoxygenation).

Freshness

Last updated 2026-06-02 04:21:34; freshness should be verified.