Rhomboid Protease Analysis in Cryptosporidium Parvum Parasite

Three rhomboid proteases (CpRom1, CpRom2, CpRom3) in the Cryptosporidium parvum parasite were analyzed for phylogeny, structure, and sporozoite localization. The study, authored by Ilaria Vanni, identifies 10 candidate substrate proteins and compares rhomboid presence across Cryptosporidium species. The dataset was last updated in April 2026.

Use Cases

• Compare phylogenetic origin and structural motifs of CpRom1, CpRom2, and CpRom3 to orthologs from Toxoplasma gondii and Plasmodium falciparum.
• Analyze subcellular localization patterns of the three rhomboids in sporozoite cells, such as apical complex or posterior pole accumulation.
• Investigate the genomic absence of PARL-like rhomboid genes in intestinal Cryptosporidium species versus gastric species.
• Identify candidate substrate proteins for C. parvum rhomboids based on similarity to known apicomplexan substrates and co-localization evidence.

Strengths

• Provides detailed analysis of three specific rhomboid proteases (CpRom1, CpRom2, CpRom3).
• Includes comparative phylogenetic analysis across multiple Cryptosporidium species and other apicomplexans.
• Identifies 10 candidate substrate proteins for functional investigation.

Limitations

• Dataset is a single 188.5 KB PDF document, offering limited scope and no raw structured data.
• Analysis is specific to Cryptosporidium parvum, limiting direct applicability to other organisms.
• Sample size for cellular localization is not quantified, potentially limiting statistical power.

Provenance

Source

Figshare, authored by Ilaria Vanni.

Collection Method

Computational phylogenetic and structural motif analysis combined with experimental cellular localization study.

Time Range

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Freshness

Last updated in April 2026.

Geography

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