Ocimum Sanctum Nanoemulsion Metabolomic and Bioactivity Data

Surfacing results from a study characterizing an Ocimum sanctum (Holy Basil) nanoemulsion, including physicochemical properties and multi-bioactivity assays. It includes data on droplet size (51–73 nm), cytotoxicity IC50 values against four cancer cell lines (13–25 μg/mL), and antioxidant activity (SC50 = 16.4 μg/mL). The data was generated by Mervat S. Mohamed and published in 2026.

Use Cases

• Analyze the relationship between nanoemulsion droplet size, polydispersity index (PDI), and cytotoxicity IC50 values across Caco-2, HepG2, MDA-MB-231, and A549 cell lines.
• Model the biphasic release profile data to predict compound delivery kinetics over an eight-day period.
• Use network pharmacology predictions (e.g., targets AKT1, STAT3) to correlate identified metabolites with experimentally validated anti-inflammatory cytokine (TNF-α, IL-6) suppression.
• Compare antibacterial inhibition zone measurements (up to 14.8 mm) and antioxidant SC50 values between the nanoemulsion and crude extract.

Strengths

• Includes specific physicochemical measurements: droplet size of 51–73 nm, PDI of 0.264, and zeta potential of -42.1 mV.
• Reports quantitative bioactivity results, including IC50 values for four cancer cell lines and one normal cell line.
• Integrates multiple analytical methods: metabolomic profiling (GC–MS, LC–QTOF–MS/MS), biological assays, and network pharmacology.

Limitations

• Dataset is presented in a single 15.6 MB PDF, limiting direct computational analysis of underlying tabular data.
• The study is described as preliminary, with conclusions noting the need for more extensive in vitro and in vivo validation.
• Sample size and number of experimental replicates for the reported measurements are not specified.

Provenance

Source

figshare, authored by Mervat S. Mohamed.

Collection Method

Data generated from laboratory experiments including nanoemulsion preparation, physicochemical characterization, and in vitro bioactivity assays.

Freshness

Last updated in March 2026.