Isoviolanthin Effects on IL-1β-Stimulated Rat Chondrocytes

A dataset from a 2026 study by Jian-Li Yin, investigating the anti-inflammatory effects of isoviolanthin on primary rat chondrocytes. The 282.3 KB Excel file contains results from network pharmacology, molecular docking, and in vitro assays including Western blot, RT-qPCR, and immunofluorescence. It was published under a CC-BY-4.0 license on figshare.

Use Cases

• Validate network pharmacology predictions for osteoarthritis drug targets based on the 141 overlapping targets identified.
• Analyze the effect of isoviolanthin on inflammatory mediator expression (e.g., CXCL3, IL-6, TNF-α) in an in vitro chondrocyte model.
• Study the relationship between isoviolanthin treatment and extracellular matrix metabolism markers (e.g., collagen II, ACAN, MMPs).
• Investigate the modulation of PI3K-AKT and MAPK signaling pathway proteins by a natural flavonoid glycoside.
• Examine the correlation between RXFP1 expression and inflammatory/catabolic responses in chondrocytes.

Strengths

• Data is derived from multiple experimental methods including network pharmacology, molecular docking, and three distinct in vitro assays (Western blot, RT-qPCR, immunofluorescence).
• Specific experimental conditions are provided, such as the use of 25 μM isoviolanthin on IL-1β-stimulated primary rat chondrocytes for 24 hours.
• The dataset is openly licensed (CC-BY-4.0), facilitating reuse and redistribution.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for statistical analysis.
• Data is from a single in vitro study using rat cells; translational relevance to human osteoarthritis requires further validation as noted in the conclusion.

Provenance

Source

Jian-Li Yin via figshare.

Collection Method

Generated through network pharmacology, molecular docking, and in vitro experiments on primary rat chondrocytes.

Freshness

Last updated 2026-05-22 11:16:23; freshness should be verified.