Data Sheet 3_Laboratory tests for bovine respiratory bacteria and antimicrobial resistance

Data Sheet 3 contains results from a study evaluating diagnostic tests for bovine respiratory disease (BRD) pathogens and antimicrobial resistance (AMR) in commercial feedlot cattle. The dataset, authored by Simon J. G. Otto and last updated in May 2026, includes results from 760 nasopharyngeal swabs analyzed via culture, long-read metagenomic sequencing, and recombinase polymerase amplification (RPA). This work provides performance estimates for these laboratory tests to support antimicrobial stewardship in beef production.

Use Cases

• Compare diagnostic test performance (sensitivity, specificity) for BRD pathogens based on the described Bayesian latent class model analysis.
• Analyze patterns of antimicrobial resistance gene detection across different testing methodologies mentioned in the description.
• Evaluate the detection of specific bacteria like Mannheimia haemolytica and Mycoplasmopsis bovis across the early feeding period and age cohorts as described.
• Assess the predictive value of different tests (AST, metagenomics, RPA) for AMR surveillance as discussed in the results.

Strengths

• Includes results from 760 respiratory samples, representing a large-scale field evaluation.
• Compares three distinct diagnostic methodologies: culture/AST, long-read metagenomic sequencing, and recombinase polymerase amplification.
• Provides specific performance metrics, such as sensitivity below 65% for most organisms and specificity above 90%.
• Released under a permissive CC-BY-4.0 license, facilitating reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale modeling.
• Data is specific to western Canadian beef feedlots, which may limit generalizability to other regions or production systems.

Provenance

Source

Simon J. G. Otto via figshare.

Collection Method

Nasopharyngeal swabs were collected from commercial feedlot calves as part of Canadian surveillance and analyzed using culture, sequencing, and molecular tests.

Freshness

Last updated 2026-05-20 05:23:54; freshness should be verified.

Geography

Samples were collected from western Canadian beef feedlots.