Proteomic Analysis of Dimorphic Sperm in Pieris rapae

Pieris rapae sperm proteomics data identifies over 1,600 proteins using high-resolution mass spectrometry. The dataset compares protein abundances between fertilizing eupyrene and non-fertilizing apyrene sperm morphs. It was created by Melissa Plakke and published in 2026.

Use Cases

• Perform differential abundance analysis on proteins enriched in eupyrene sperm linked to ion transport and vacuolar acidification.
• Compare mitochondrial and respiratory function protein profiles in apyrene sperm.
• Conduct comparative homology analyses of sperm proteins with other Lepidoptera species like Danaus plexippus and Manduca sexta.
• Investigate the evolutionary dynamics of sperm proteomes based on shared homologous protein data.

Strengths

• Identifies more than 1,600 proteins, nearly doubling previous reports for other lepidopteran species.
• Provides distinct proteomic profiles for two sperm morphs (eupyrene and apyrene).
• Includes comparative analyses with two other Lepidoptera species (Danaus plexippus and Manduca sexta).

Limitations

• Dataset is very small (19.7 KB), indicating limited scope and likely a summary or processed results table rather than raw spectral data.
• Unknown row and column structure limits direct analytical applications without further inspection.
• Focus is on a single model species (Pieris rapae), limiting generalizability across Lepidoptera.

Provenance

Source

Melissa Plakke via figshare.

Collection Method

Proteomic characterization using high-resolution, label-free mass spectrometry.

Time Range

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Freshness

Last updated March 2026.

Geography

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