SLST-Seq: Strain-Level Typing Data for Cutibacterium acnes from Skin Samples

SLST-Seq is a culture-independent method for strain-level profiling of the skin bacterium Cutibacterium acnes. The dataset, shared by Elody Orcel on figshare, contains results validated using single-isolate controls, genomic DNA mixtures, and spike-in dilution series. It provides a framework for analyzing C. acnes population structure directly from low-input skin-strip samples.

Use Cases

• Strain-level profiling of C. acnes based on the single-locus sequence typing (SLST) marker.
• Analyzing inter-individual variability in skin microbiome composition from healthy volunteers.
• Validating quantitative accuracy of microbiome sequencing methods across target-to-background DNA ratios.
• Studying the conservation of donor-specific community structures between different skin sites.

Strengths

• Method validated for high specificity and strong run-to-run reproducibility.
• Demonstrated quantitative accuracy across a wide range of target-to-background DNA ratios.
• Licensed under CC-BY-4.0 for open use.

Limitations

• Dataset is very small (8.6 KB), indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

SLST-Seq, a culture-independent approach combining unique molecular identifier barcoding with de novo assembly.

Freshness

Last updated 2026-05-20 10:46:11; freshness should be verified.