Data Sheet 4_Comparative analysis of the rhizosphere microbiome and transcriptome in clubr

29.6 KB of data comparing soil properties, microbial communities, and gene expression between clubroot-susceptible (HYZ62, disease index 54.86) and resistant (HYZ5R, disease index 17.05) rapeseed varieties. The dataset, authored by Jingjing Liao and last updated on 2026-04-21, includes measurements of electrical conductivity, alkali-hydrolysable nitrogen, relative abundances of microbial genera, and expression levels of defense-related genes.

Use Cases

• Identify microbial taxa associated with disease resistance based on relative abundance data for genera like Burkholderia-Caballeronia-Paraburkholderia and Trichoderma.
• Analyze differential gene expression patterns for immunity-related genes such as CML, WRKY, PR1, RIN4, RPS5, and HSP90 between resistant and susceptible varieties.
• Correlate soil physicochemical properties, like electrical conductivity and alkali-hydrolysable nitrogen content, with plant health outcomes after pathogen infection.
• Investigate the suppression of defense-related secondary metabolic pathways, such as phenylpropanoid biosynthesis, in response to Plasmodiophora brassicae infection.

Strengths

• Includes specific quantitative disease indices (54.86 for susceptible HYZ62, 17.05 for resistant HYZ5R) for clear phenotype comparison.
• Reports concrete fold-changes in soil electrical conductivity (1.73x and 1.57x higher in resistant variety) and percentage changes in microbial abundance (30.36% decrease in Bacillus).
• Compares multiple data layers: soil physicochemical properties, rhizosphere microbiome composition, and host plant transcriptomic responses.

Limitations

• Row count is unknown, which may limit suitability assessment for statistical modeling.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (29.6 KB), indicating limited scope, likely summarizing aggregated results rather than raw sequence or measurement data.

Provenance

Source

figshare, author Jingjing Liao.

Collection Method

Likely generated from controlled experiments comparing inoculated and uninoculated treatments of two rapeseed varieties, involving soil analysis, microbiome sequencing, and RNA sequencing.

Freshness

Last updated 2026-04-21 05:31:31; freshness should be verified.

Geography

The study addresses a threat to the rapeseed industry in China, but specific sample collection geography is not stated.