ant(9)-Ie: Novel Spectinomycin Resistance Gene in Providencia rettgeri P17

A novel resistance gene, ant(9)-Ie, was identified in Providencia rettgeri P17, isolated from animal farm wastewater. The gene confers a 64-fold increase in spectinomycin MIC and has a catalytic efficiency of (8.22 ± 1.24) × 10² M⁻¹·s⁻¹. This dataset, a 239.8 KB PDF published by Lulu Huang under CC-BY-4.0, documents the gene's discovery and characterization.

Use Cases

• Comparative genomic analysis based on the whole-genome sequencing data described.
• Phylogenetic analysis of resistance gene backgrounds based on the conserved genetic environments mentioned.
• Kinetic modeling of enzyme activity based on the reported spectinomycin catalytic efficiency parameters.
• Studying the distribution of resistance genes based on the finding that ant(9)-Ie homologs were found only in Providencia strains.

Strengths

• Includes specific kinetic parameters for the ANT(9)-Ie enzyme, such as a spectinomycin catalytic efficiency of (8.22 ± 1.24) × 10² M⁻¹·s⁻¹.
• Documents a 64-fold increase in the Minimum Inhibitory Concentration (MIC) of spectinomycin conferred by the novel gene.
• Provides comparative genomic and phylogenetic analysis of the resistance gene's background.

Limitations

• Dataset is a single 239.8 KB PDF document, which is a very small and limited data scope.
• Row count and column-level documentation are absent; data must be extracted manually from the text.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

Providencia rettgeri P17 isolated from animal farm wastewater.

Collection Method

Isolation by plate streaking, MIC determination by agar dilution, gene function elucidated via molecular cloning and heterologous expression, genome analysis via whole-genome sequencing.

Freshness

Last updated 2026-06-02 04:28:45; freshness should be verified.