Genomic Analysis of Antifungal Streptomyces Strain STM-2 from Taiwan

Laying out a genomic analysis of Streptomyces melanosporofaciens strain STM-2, isolated from Chiayi County, Taiwan. The study includes a whole-genome sequence revealing 52 predicted biosynthetic gene clusters (BGCs) and identifies genes for antifungal enzymes like chitinases and proteases.

Use Cases

• Identify and analyze the 52 predicted biosynthetic gene clusters (BGCs) for type I and II PKS, NRPS, and hybrid systems.
• Examine genes for antifungal hydrolytic enzymes, including chitinases, β-1,3-glucanases, xylanases, pectinases, and proteases, to understand biocontrol mechanisms.
• Use the high-resolution Average Nucleotide Identity (ANI) and digital DNA–DNA hybridization (dDDH) scores for comparative pangenomic analysis of Streptomyces strains.
• Investigate the accessory genome containing unique genes associated with specialized antifungal activity through evolutionary analysis.
• Utilize the provided AlphaFold2 protein models with high pLDDT scores for in silico structural analysis of unique gene products.

Strengths

• Genome mining predicted an extensive repertoire of 52 biosynthetic gene clusters (BGCs).
• Comparative analysis is supported by high-resolution Average Nucleotide Identity (ANI) and digital DNA–DNA hybridization (dDDH) scores.
• In silico structural analysis provides three-dimensional protein models generated by AlphaFold2 with high pLDDT scores.

Limitations

• The dataset is a single 816.0 KB DOCX file, which is a small document format not structured as a typical tabular or genomic data file.
• No raw genomic sequence data, columnar data, or sample data is provided, limiting direct computational analysis.
• The analysis is based on a single novel strain (STM-2), limiting broader comparative or population-level insights.

Provenance

Source

figshare, authored by Muhammed Opoku Gyamfi.

Collection Method

Integrated approach involving physiological/biochemical characterization, genome sequencing, genome mining with antiSMASH, comparative genomics, and evolutionary analysis.

Freshness

Last updated on 2026-03-20.

Geography

Chiayi County, Taiwan.