Laron Mouse Liver RNA-seq Data: Gene Therapy Response and Sex-Bias Effects

RNA sequencing data from male and female mouse livers, comparing wild-type, GHR-deficient (Laron), and gene therapy-treated groups. The dataset, authored by Joshua K. Tay and shared under CC-BY-4.0, includes expression changes for 448 genes following therapy and 2781 genes altered by GHR loss. It was last updated on 2026-05-28.

Use Cases

• Analyze differential gene expression in response to liver-specific GH receptor gene therapy based on the 448 significantly changed genes.
• Investigate sex-biased gene regulation in the liver based on the enrichment of sex-biased genes among dysregulated genes.
• Compare the efficacy of gene therapy to wild-type levels based on Igf1 and Igfals expression reaching 40–45% of normal.
• Identify potential oncogenic risks from gene therapy based on the upregulation of proto-oncogenes like Ascl1 and Tmprss4 beyond wild-type levels.

Strengths

• Includes a control group treated with Luciferase vector, showing minimal effect with only 4 differentially expressed genes.
• Quantifies the scale of expression changes with 448 genes altered by therapy and 2781 by GHR loss.
• Reports specific fold-changes for key IGF signaling genes (Igf1, Igfals ~16-fold).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (87.6 KB), indicating limited scope, likely summary statistics rather than raw sequence reads.

Provenance

Source

figshare

Collection Method

RNA sequencing performed on mouse livers from genetically modified and treated groups.

Freshness

Last updated 2026-05-28 04:56:28; freshness should be verified.