Data Sheet 4_Integrated transcriptomics identifies ER stress–associated apoptosis in post-

A research document details integrated bulk and single-cell RNA sequencing analyses of acute kidney injury following traumatic cardiac arrest in a porcine model. The study, authored by Kechun Zhou and posted on figshare, investigates the renoprotective effects of Dl-3-n-butylphthalide (NBP) and its association with endoplasmic reticulum stress and apoptosis pathways. The document was last updated on June 4, 2026.

Use Cases

• Study endoplasmic reticulum stress and unfolded protein response pathways in kidney injury based on integrated transcriptomic analyses.
• Investigate the pharmacological effects of Dl-3-n-butylphthalide on acute kidney injury biomarkers like serum creatinine and blood urea nitrogen.
• Analyze cell-state-specific apoptosis in proximal tubule cells using single-cell RNA sequencing and pseudotime inference methods.
• Validate in-vivo transcriptomic findings with histopathological and protein expression data from a porcine model.

Strengths

• Integrates multiple high-resolution data modalities including bulk transcriptomics, microarray, and single-cell RNA sequencing.
• Includes in-vivo validation with specific measurements (e.g., serum creatinine, BUN at 1, 2, 4, 24h) and protein expression analysis.
• Dataset is openly licensed under CC-BY-4.0, facilitating reuse.

Limitations

• Description metadata is limited; actual data quality requires manual inspection after download.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is a 1.2 MB DOCX file, which may contain processed results and textual analysis rather than raw, structured data tables.

Provenance

Source

figshare

Collection Method

Integrated analysis of public ischemia-reperfusion injury transcriptomic datasets combined with original single-cell RNA sequencing and in-vivo porcine model experiments.

Freshness

Last updated 2026-06-04 05:33:37; freshness should be verified.