Isoviolanthin Effects on IL-1β-Stimulated Rat Chondrocytes: In Vitro Assay Data

A dataset from figshare, authored by Jian-Li Yin and last updated on 2026-05-22, containing results from an in vitro study of knee osteoarthritis. The 33.5 KB Excel file likely contains experimental measurements from primary rat chondrocytes treated with interleukin-1β and the flavonoid isoviolanthin. The data supports findings on inflammatory mediators, extracellular matrix metabolism, and signaling pathway activation.

Use Cases

• Validate network pharmacology predictions based on identified gene targets like AKT1 and MAPK1.
• Analyze the dose-response relationship of isoviolanthin based on the described 25 μM working concentration.
• Model the inhibition of PI3K-AKT and MAPK signaling pathways based on Western blot and immunofluorescence data.
• Correlate expression levels of inflammatory mediators (e.g., IL-6, TNF-α) with matrix-degrading enzymes (e.g., MMP13).

Strengths

• Data is associated with a detailed methodological description including network pharmacology (141 overlapping targets) and in vitro validation.
• The experimental conditions, such as the 25 μM concentration and 24-hour treatment, are explicitly defined.
• Results are linked to specific biological pathways (PI3K-AKT, MAPK) and markers (collagen II, MMPs, RXFP1).

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small at 33.5 KB, indicating a limited experimental scope.

Provenance

Source

figshare

Collection Method

In vitro assays on primary rat chondrocytes, supported by network pharmacology and molecular docking.

Freshness

Last updated 2026-05-22 11:16:23; freshness should be verified.