Immunoprofiling of T-Cell Responses in IGRA/TST-Negative Subjects

54 IGRA/TST-negative subjects were studied using flow cytometry to profile T-cell responses to mycobacterial antigens. The research, authored by Balaji Pathakumari and published on figshare in 2026, found that specific T-cell activation markers could differentiate between potentially exposed and unexposed individuals. This suggests a potential method for detecting Mycobacterium tuberculosis exposure missed by standard tests.

Use Cases

• Developing diagnostic models based on flow cytometric T-cell activation profiles.
• Identifying novel immune signatures for Mtb exposure based on CD4+ and CD8+ T-cell responses.
• Validating antigen-specific immunoprofiling as a tool for tuberculosis research.
• Comparing immune response patterns between exposed and unexposed control groups.

Strengths

• Includes data from 54 human subjects, with 27 categorized as at risk for prior Mtb exposure.
• Reports statistically significant results (P<0.05) and ROC analysis with AUCs >0.7 for certain markers.
• Published under a permissive CC-BY-4.0 license for open reuse.

Limitations

• Row and column counts are unknown, limiting assessment of dataset scale and structure.
• The dataset is small (85.6 KB), indicating limited scope.
• Data is presented in a PDF format, which may require extraction to be usable for analysis.

Provenance

Source

figshare

Collection Method

Flow cytometric multiparameter profiling of T-cell activation markers in peripheral blood samples.

Freshness

Last updated 2026-05-01 05:25:05.