Rice GA2ox Gene Family Mutant Phenotypes from Systematic CRISPR Knockouts

A 2026 study by Kun-Ting Hsieh presents data from 36 CRISPR/Cas9-edited rice lines targeting nine OsGA2ox genes. The dataset includes single-, double-, triple-, and quadruple-gene knockout mutants and their measured effects on agronomic traits like plant height, grain number, and fertility. It provides allele-validated evidence for functional diversification and hierarchical relationships within this gibberellin-regulating gene family.

Use Cases

• Identify dominant and minor gene paralogs based on mutant phenotype comparisons described in the results.
• Model hierarchical regulatory frameworks within multigene families using the combinatorial knockout data.
• Investigate trade-offs between agronomic traits like plant height and grain fertility using the quadruple mutant data.
• Study functional redundancy and specialization in hormone signaling pathways based on the systematic mutant analysis.

Strengths

• Systematic generation of 36 CRISPR-edited mutant lines targeting all nine genes in the family.
• Analysis includes combinatorial knockouts (double, triple, quadruple mutants) to assess redundancy.
• License is CC-BY-4.0, allowing for broad reuse and sharing.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small in scale at 166.8 KB, indicating limited raw data volume.

Provenance

Source

figshare

Collection Method

Data generated from CRISPR/Cas9 gene editing experiments and phenotypic analysis of rice mutants.

Freshness

Last updated 2026-05-07 05:42:20; freshness should be verified.